8:00 AM - 10:15 AM

Introduction to Microscopy (Sampling, Slide Preparation, Wastewater Microbiology, The Role of Bacteria, Growth Characteristic in Activated Sludge & Parameters Affecting Their Growth)

10:15 AM - 10:30 AM

Break

10:30 AM - 12:00 PM

The Role of Protozoa and Metazoans, Parameters Affecting Their Growth & Identifying Protozoa and

Metazoans

12:00 PM - 1:00 PM

Lunch

1:00 PM - 2:30 PM

Process Control Tools Using the Microscope, Dispersed Bacteria, Protozoa & Metazoa: Relative Dominance, Slime Bulking & Nutrient Deficiency, India Ink Staining & Toxicity

2:30 PM - 2:45 PM

Break

2:45 PM - 4:00 PM

Filamentous Bacteria Identification & Control, Identifying Bulking Filamentous Bacteria, Identifying Foaming, Filamentous Bacteria, Monitoring and Process Control & Gram Staining For Filamentous Bacteria

8:00 AM - 10:00 AM

Biological Phosphorus Removal Process Description, Microbial Activity in the Anaerobic Zone, Hydrolysis & Fermentation, Phosphorus Accumulating Organisms (PAOs), Glycogen Accumulating Organisms (GAOs) Microbial Activity in the Aerobic Zone, Luxury Phosphorus Uptake & GAOs in the Aerobic Zone

10:00 AM - 10:15 AM

Break

10:15 AM - 12:00 PM

Factors Affecting PAO Performance, Carbon & Volatile Fatty Acids (VFAs), PAOs vs GAOs, Wastewater Composition, pH, Solids & Hydraulic Retention Time, Mixed Liquor Concentration & Cations

12:00 PM - 1:00 PM

Lunch

1:00 PM - 2:00 PM

Microbiological Monitoring, Neisser Staining, Sudan Black (PHB) Staining & Quantifying PAOs and GAOs

2:15 PM - 2:30 PM

Break

2:30 PM - 4:00 PM

Nitrification Basics, Conditions Affecting Nitrification, Denitrifications & Balancing Bio P and Nitrification Systems